Figure 6.

Details of in vitro shoot organogenesis in ‘Delite’ rabbiteye blueberry using WPM culture medium supplemented with TDZ. Different views of shoots and details under scanning electron microscopy–SEM (a–f) and light microscopy (g–l). (a) Newly formed adventitious small shoots (black arrow) on the surface of the three-week-old leaf explant culture. (b) Adventitious shoot (gray arrow) on a four-week-old leaf explant culture, showing leaf primordia (abaxial side visible). (c) Adventitious shoot (white arrow) on a four-week-old leaf explant culture, showing foliar primordia with stomata (black circle) and trichomes (white circle). Abaxial and adaxial surfaces of the leaf primordia visible. (d) Stomata on the abaxial surface of the leaf primordium: ostiole surrounded by guard cells. (e) Adaxial surface of the leaf primordium showing no stomata. (f) Trichomes on the leaf primordium (stars). (g) Adventitious shoots at three weeks of culture: shoot apical meristem (in detail), leaf primordium, and callus. (h) Adventitious shoot with leaf primordium and shoot apical meristem at four weeks of culture. (i) Detail of the adventitious shoot, showing shoot apical meristem, leaf primordium, procambium (detail), vascular tissue (detail), and trichomes (white circles) at four weeks of culture. (j) Leaf primordium formation at four weeks of culture. (k) Adventitious shoot showing the formation of vascular tissue (white ar) at four weeks of culture (l) Adventitious shoot with shoot apical meristem, leaf primordia with protoderm (detail), procambium (black ar), vascular tissue (detail) at 4 weeks of culture, and callus tissue. Abbreviations: Ab, abaxial; Ad, adaxial; Ca, callus; Ex, explant; GC, guard cell; LP, leaf primordium; Os, ostiole; Pc, procambium; Pd, protoderm; SAM, shoot apical meristem; Sh, shoot; TDZ, thidiazuron; Tr, trichome; VT, vascular tissue; WPM, woody plant medium.