Table 3.

Effects of two explant orientations (adaxial or abaxial side in contact with the medium) and two leaf portions (basal or apical) on in vitro shoot organogenesis in ‘Delite’ rabbiteye blueberry using WPM culture medium supplemented with TDZ.

Explant orientation	Leaf portion
Survival rate (%)	Basal	Apical	Mean
Adaxial	100.0 ± 0.0	100.0 ± 0.0	100.0 a
Abaxial	82.0 ± 10.3	94.0 ± 4.5	88.0 b
Mean	91.0 A	97.0 A	94.0
Shoot regeneration rate (%)
Adaxial	94.0 ± 4.5	100.0 ± 0.0	97.0 a
Abaxial	74.0 ± 12.0	94.0 ± 4.5	84.0 b
Mean	84.0 B	97.0 A	90.5
Number of shoots formed/explant (total)
Adaxial	59.2 ± 15.4 aA	35.8 ± 3.8 aA	47.5
Abaxial	22.8 ± 3.1 bA	41.3 ± 9.6 aA	32.0
Mean	41.0	38.6	39.8
Number of shoots formed/explant (large sized)
Adaxial	4.1 ± 0.7	4.5 ± 1.5	4.3 a
Abaxial	2.5 ± 0.5	1.1 ± 0.2	1.8 b
Mean	3.3 A	2.8 A	3.1
Number of shoots formed/explant (medium sized)
Adaxial	9.0 ± 1.1	6.4 ± 1.1	7.7 a
Abaxial	4.0 ± 0.6	6.1 ± 1.8	5.1 a
Mean	6.5 A	6.3 A	6.4
Number of shoots formed/explant (small sized)
Adaxial	46.1 ± 15.3 aA	24.9 ± 4.3 aA	35.5
Abaxial	16.2 ± 3.0 bA	39.5 ± 7.6 aA	27.9
Mean	31.2	32.2	31.7

The results are presented as the mean ± standard error (SE). Means followed by different lowercase letters in the same column and by different uppercase letters in the same horizontal line differ statistically at 5% of Tukey's multiple range tests. Abbreviations: TDZ, thidiazuron; WPM, woody plant medium.