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. 2020 Nov 20;80(6):1092–1103.e4. doi: 10.1016/j.molcel.2020.11.025

Figure 1.

Figure 1

SARS-CoV-2 N Protein Forms Biomolecular Condensates in the Presence of RNA

(A) Top: schematic of N protein domain architecture. NTE, N-terminal extension; NTD, N-terminal domain; SR, SR region; CTD, C-terminal domain; CTE, C-terminal extension; CBP, CTD basic patch. Bottom: features of amino acid sequence. PLAAC, prion-like amino acid composition (Lancaster et al., 2014); NCPR, net charge per residue. See Figure S1 for sequence.

(B) Light microscopy images of N protein condensates after a 30-min incubation at room temperature with the indicated RNA molecules. All images are representative of multiple independent experiments; scale bar, 10 μm.

(C) Condensate formation by N protein (10 μM) over a range of 5′-400, TRS-10, and Random-10 RNA concentrations. All images are representative of multiple independent experiments; scale bar, 10 μm. Random-10 RNA is a mixed population of 10-nt random sequences. See Figure S3 for condensate formation at other N protein and 5′-400 RNA concentrations.