Fig. 3.

Ferroptosis markers in fibroblasts of patients with FRDA. A) Lipid peroxides detected by using the fluorescent lipophilic Bodipy-C11 sensor (in green the oxidized lipids, in red the total lipids, scale bar = 100 μm). B) Representative Western blot (right) and densitometric analysis (left) of NRF2, and GPX4 protein levels in FRDA fibroblasts. Vinculin has been used as loading control. C) Immunofluorescence of mitochondrial network by using the anti-TOM20 antibody. As positive ferroptosis control, fibroblasts have been treated with 0.2 μM ferroptosis inducer RSL-3 for 24 h. Scale Bar = 25 μm. Three independent experiments were performed on fibroblasts obtained from n = 2 FRDA patients and values were expressed as mean ± SD. *p < 0.05, **p < 0.01, compared to controls' cells, and ^#p < 0.05, ^##p < 0.01, compared to treated cells (by Student's two-tailed t-test). (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)