Table 4.
Primer sequences used for mRNA quantification in HUVECs following treatment with the α-TTP and γ-TTP monomers in presence or absence of a cytokine mix.
| Target primer | Forward primer | Reverse primer | Annealing Temp. (∘C) |
|---|---|---|---|
| CCL2 | CAGCCAGATGCAATCAATGC | GCACTGAGATCTTCCTATTGGTGAA | 59 |
| COX2 | CATGGGGTGGACTTAAATCAT | TCTTTGACTGTGGGAGGATACA | 57 |
| GAPDH | CAATGACCCCTTCATTGACC | GATCTCGCTCCTGGAAGATG | 58 |
| HPRT1 | TGGCGTCGTGATTAGTGATG | CTCGAGCAAGACGTTCAGTC | 60 |
| ICAM1 | CTGGCGGTTATAGAGGTACG | GTGACCGTGAATGTGCTCTC | 59 |
| IL6 | TGACAAACAAATTCGGTACATCCT | TCTGCCAGTGCCTCTTTGCT | 58 |
| TNF | GGTTTGCTACAACATGGGCTACA | CCCCAGGGACCTCTCTCTA | 59 |
CCL2: C-C motif chemokine ligand 2; COX2: PTGS2 prostaglandin-endoperoxide synthase 2 (prostaglandin G/H synthase and cyclooxygenase); GAPDH: Glyceraldehyde-3-phosphatebdehydrogenase; HPRT1: Hypoxanthine-phosphoribosyl-transferase 1; ICAM1: intercellular adhesion molecule 1; IL6: Interleukin 6; TNF: TNF tumor necrosis factor (TNF superfamily, member 2); TTP: tocopherol transfer protein.