Fig. 2.

Evodiamine decreases PD-L1 and MUC1-C expression in NSCLC cells. a-d After H1975 and H1650 cells were treated with of evodiamine for 24 h, the mRNA expression of MUC1-C and PD-L1 is determine by quantitative RT-PCR. e-f H1650 and H1975 cells were treated with evodiamine for 24 h, and the membrane surface PD-L1 expression is detected by flow cytometry. i-j H1650 and H1975 cells are treated with different concentration of evodiamine for 24 h and western blot assays are to analyze MUC1-C and PD-L1 for 24 h. GAPDH is used as a loading control. The results are presented in three independent experiments with the mean ± S.D. (n = 3, *P < 0.05; **P < 0.01; ***P < 0.001). a: isotryposin control, b: control, c: 2 μM evodiamine, d:4 μM evodiamine, e: 8 μM evodiamine, f: 16 μM evodiamine