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. 2020 Nov 19;39:249. doi: 10.1186/s13046-020-01741-5

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 7 — Combination of evodiamine and PD-1 mAb treatment enhance tumor growth control and survival of the Lewis lung carcinoma model. a The survival rate of LLC and the combination of evodiamine and PD-1 mAb improves the survival of the mice. b The body weight of mice does not change. Tumor volume is checked at the beginning of the treatment period. The combination evodiamine and PD-1 mAb treatment enhanced inhibits tumor growth in the Lewis lung carcinoma model (c-e), The efficacy of anti-tumor growth is estimated by measuring tumor volume (c), tumor size (d) and tumor weight (e). h Lymphocytes cells are analyzed using flow cytometry with FIN-γ, TNFα and Granzyme B, CD4 and CD8 antibodies. The results were presented in three independent experiments with the mean ± S.D. (n = 3, *P < 0.05; **P < 0.01; ***P < 0.001)