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. 2020 May 15;66(4):1195–1211. doi: 10.1007/s10620-020-06319-x

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License, which permits any non-commercial use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder.To view a copy of this licence, visit http://creativecommons.org/licenses/by-nc/4.0/.

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Fig. 4 — Effect of zinc on the tight junctional protein, CLAUDIN-7, and the VEGF receptor protein, VEGF-R2, in biopsy tissue samples confirmed histologically as secretory intestinal metaplasia. Densitometry of Western blot bands for the proteins, CLAUDIN-7 and VEGF-R2, expressed as vertical point plots. Each data point represents the Western blot band density obtained from PAGE and immunoblots performed on whole cell lysates of biopsy tissue from a single patient. Patients were zinc-treated or placebo-treated as described in Methods, but the patient groups here consisted only of BE cases confirmed histologically as secretory intestinal metaplasia. P values represent Student’s t tests, one-tailed, n = 5. Densitometry conducted on a Memcode stain of total protein served as the loading control for each band (lane)