Figure 6.

Hematopoietic knockout of CXCL4 leads to reduced expression of profibrotic pathways and reduced inflammation. (A) Schematic of cell populations analyzed. CD41⁺ MKs are either WT or CXCL4 knockout (GFP⁺) and transduced with either ThPO-inducing fibrosis or EV as a control. tdTom⁺ stromal cells were exposed to CXCL4 expressing or knockout hematopoietic cells/MKs in control or fibrosis. Heatmap representation of PROGENy analysis of sort-purified MKs and Gli1⁺ stromal cells from WT ThPO or Cxcl4^−/− ThPO mice compared with EV control mice. (B) Heatmap representation of specific PROGENy pathways such as JAK-STAT, NF-κB, and TNF-α. (C) Heatmap representation of Discriminant Regulon Enrichment Analysis of transcription factors of sort-purified MKs and Gli1⁺ stromal cells from WT ThPO or Cxcl4^−/− ThPO mice compared with EV controls.