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. 2020 Nov 19;21:809. doi: 10.1186/s12864-020-07226-1

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 6 — Expression profiles of 17 NBS-encoding genes in four pear accessions at enlarged stage by RT-qPCR analysis and RNA-seq data. The relative expression levels of each individual NBS-encoding genes were normalized by the Pyrus GAPDH gene. The expression level of PyW14 sample was used as a reference (relative expression level = 1). The letter “r” represents the correlation between RNA-seq data and RT-qPCR data. Bars represent mean relative expression level of genes (rose red: PyW14, purple: PyW13, blue: PyL2, cyan: PyI1); lines represent value of RPKM. Mean relative expression value and corresponding standard deviation were calculated by the three replicates of relative expression value. Four pears included Asian wild (PyW13,14) and cultivated (PyL2,PyI1) accessions. Left y-axis represents the relative expression level and right y-axis represents RPKM values