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. 2020 Nov 19;21:809. doi: 10.1186/s12864-020-07226-1

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 7 — Divergence of Pbr025269.1 between Asian cultivated and wild groups. a One significantly different non-synonymous SNP located on NBS domain. b Genotyping distribution of SNP1 in Asian cultivated and wild groups (chi-square test, p-value = 1.42E-08). c Ka/Ks ratio and nucleotide diversity of Pbr025269.1 in Asian wild and cultivated groups. Bars mean nucleotide diversities and spots mean Ka/Ks ratios. d Expression profiles of Pbr025269.1 in ten Asian cultivated (PyL2, PyL7, PyL8, PyL9, PyL10, PyL11, PyI1, PyI9, PyI11, PyI14) and seven Asian wild (PyW5, PyW6, PyW7, PyW9, PyW12, PyW13, PyW14) pear accessions at enlarged stage by RT-qPCR analysis. The relative expression levels of each individual NBS-encoding genes were normalized by the Pyrus GAPDH gene. The expression level of PyW14 sample was used as a reference (relative expression level = 1). Each point means relative expression level of one pear accession. ‘**’ means significant difference (T-test, p-value< 0.05) between wild and cultivated groups. (e) RPKM value of Pbr025269.1 in P. calleryana and ‘hongfen’ pear (an Asian cultivate pear), ‘Treat’ means inoculated A. alternata groups and ‘CK’ means control groups; red represents P. calleryana, blue represents ‘hongfen’(P. pyrifolia)