Table 2.

Frequency of cis-elements identified in the promoter regions of NBS-encoding genes in two pear species

Cis-element name	Sequence	Putative response	Frequencya
			‘Dangshansuli’	‘Bartlett’
CAAT-box	CCAAT	Seed	1.00	1.00
TATA-box	TATAA	core promoter element	1.00	1.00
MYC	CATTTG	Cold	0.95	0.96
ARE	AAACCA	anaerobium	0.75	0.74
STRE	AGGGG	Stress	0.72	0.73
ABRE	ACGTG	ABA	0.69	0.74
Box 4	ATTAAT	Light	0.69	0.72
CGTCA-motif	CGTCA	MeJA	0.67	0.75
TGACG-motif	TGACG	MeJA	0.67	0.75
TCT-motif	TCTTAC	Light	0.48	0.51
GT1-motif	GGTTAA	Light	0.49	0.46
MBS	CAACTG	Drought	0.49	0.52
ERE	ATTTCATA	Ethylene	0.46	0.49
W box	TTGACC	phytochrome down-regulation	0.43	0.45
LTR	CCGAAA	low-temperature	0.41	0.46
TCA-element	CCATCTTTTT	salicylic acid	0.39	0.49

^aFrequency = Genes (containing this cis-element)/All NBS-encoding genes in Dangshansuli (Bartlett). Cis-elements on upstream 1500 bp sequence of NBS genes were identified by Plant CARE (http://bioinformatics.psb.ugent.be/webtools/plantcare/html/) program. Genome sequences of Dangshansuli and Bartlett were downloaded from NJAU (http://peargenome.njau.edu.cn/) and phytozome database (https://phytozome.jgi.doe.gov/)