Fig. 3.

Neurons secrete Aβ that then accumulates within the vascular wall. a Aβ40 and Aβ42 levels were quantified by ELISA in the chamber and circulation media of bioengineered arterial NVU after 3 weeks. Aβ40 (b) and Aβ42 (c) levels in chamber media of tissues composed of EC and SCM (bipartite), EC, SMC and astrocyte (tripartite) and EC, SMC, astrocytes and neurons (NVU) after one or three weeks in culture. d Vascular Aβ40 and Aβ42 level in RIPA and GuHCl soluble fractions were quantified by ELISA in NVU after three weeks in culture. The correlation between the level of Aβ40 (e) and Aβ42 (f) in circulation and tissue chamber were assessed through Pearson correlation analysis. The correlation coefficient (R²) and p-value are shown in each panel. Aβ40 (g) and Aβ42 (h) vascular deposition were quantified in RIPA soluble fraction after a week (bipartite, tripartite and NVU) and three weeks (NVU) in culture. i The level of p-tau (AT8, CP13 and PHF1) was measured by Western blot in NVU and tripartite tissues and compared to total tau (DA9). Points in graphed data represent individual bioengineered vessels, bars represent mean, error bars represent ±SEM and analysed by one way ANOVA or Pearson correlation. Values below the detection of the ELISA are plotted in gray. * = p < 0.05, ** = p < 0.01, *** = p < 0.001