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. 2020 Nov 19;21:305. doi: 10.1186/s12931-020-01571-1

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 2 — The presence of cilia at the surface of the cells confirms that H292 cells were grown at the air–liquid interface (ALI). a Characterization of the ALI cell culture model. H&E staining reveals the multiple cell layers on the apical surface following 1 day of exposure to either air or butter-flavored e-cig aerosol. b Scanning electron microscopy images of representative H292 cells exposed to either air or butter-flavored e-cig aerosols for 1 day at the ALI, with a higher magnification of the cilia present at the surface of the cells. Images showed are representative for each exposure group. For air-exposed cells: a total of 38 SEM images were taken; for butter-flavored e-cig aerosol-exposed cells: a total of 53 SEM images were taken