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. 2020 Nov 5;21(1):17. doi: 10.3892/etm.2020.9449

Figure 3.

Figure 3

TMPO-AS1 knockdown inhibits breast cancer cell migration and invasion in vitro and in vivo. (A) The Transwell migration and invasion assay resulted indicated that TMPO-AS1 knockdown inhibited T47D and MCF7 cell migration and invasion. Scale bar, 20 µm. (B) The wound healing assay results suggested that TMPO-AS1 knockdown inhibited T47D and MCF7 cell migration. Scale bar, 40 µm. (C) Lung colonization of TMPO-AS1-knockdown and control MCF-7 cells was assessed using an in vivo lung metastasis model (n=5 per group). Representative lung images (upper) and hematoxylin and eosin-stained sections (lower; scale bar, 100 µm). The percentage of the lung area occupied by tumors was quantified. Data are presented as the mean ± SD of three independent experiments. *P<0.05 and **P<0.01 vs. siNC or shNC group. TMPO-AS1, TMPO antisense RNA 1; si, small interfering RNA; NC, negative control; sh, short hairpin RNA; ns, not significant.