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. 2020 Nov 5;21(1):17. doi: 10.3892/etm.2020.9449

Figure 4.

Figure 4

TMPO-AS1 is a molecular sponge for miR-140-5p. (A) miR-140-5p binding sites in TMPO-AS1 were predicted via bioinformatics analysis. (B) Transfection efficiency of miR-140-5p mimic and miR-140-5p inhibitor. (C-D) Luciferase reporter assays were performed using MCF7 cells co-transfected with TMPO-AS1-WT or TMPO-AS1-Mut reporter plasmids and (C) miR-140-5p inhibitor or (D) miR-140-5p mimic. Relative TMPO-AS1 expression levels in (E) miR-140-5p mimic- and (F) miR-140-5p inhibitor-transfected T47D and MCF7 cells. Data are presented as the mean ± SD of three independent experiments. **P<0.01 vs. corresponding NC group. TMPO-AS1, TMPO antisense RNA 1; miR, microRNA; WT, wild-type; Mut, mutant; NC, negative control; 3'UTR, 3'-untranslated regions.