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. 2020 Nov 16;12:11639–11647. doi: 10.2147/CMAR.S236188

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© 2020 Wei et al.

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LSCC sponges miR-24 to upregulate p53. UM-SCC-17A cells were transfected with IUR expression vector or miR-24 mimic to further analyze the interaction between IUR and miR-24. Over expression of IUR and miR-24 was confirmed by qPCR at 24h post-transfection (A). The effects of IUR and miR-24 overexpression on the expression of each other were also analyzed by qPCR at 24h post-transfection (B). The effects of IUR and miR-24 expression on the expression of p53 in UM-SCC-17A cells were analyzed by qPCR and Western blot at mRNA (C) and protein (D) levels, respectively. Experiments were repeated 3 times and data were expressed as mean values. *p < 0.05.