Fig. 2. Metalloproteomic maps of untreated and Cd²⁺-treated S. pneumoniae.

Detection and distribution of Zn²⁺- and Cd²⁺-associated proteins throughout the pneumococcal metalloproteome. a Zn²⁺-associated proteins in untreated S. pneumoniae, b Zn²⁺-associated proteins in Cd²⁺-treated S. pneumoniae, c Cd²⁺-associated proteins in Cd²⁺-treated S. pneumoniae. Anion exchange (AEX) separation of proteins is shown on the x-axis and size exclusion chromatography (SEC) separation of proteins (time in seconds) is shown on the y axis. The concentration of metal detected (in parts per billion [ppb]) is shown on the z-axis. All metalloproteomic maps are representative of 2 mg total cytoplasmic protein.