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. 2020 Nov 19;10:20155. doi: 10.1038/s41598-020-77110-1

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Summary of binary vectors carrying self-excisable CRISPR T-DNAs used in the present study. Vectors from 1 to 4 were used for grapevine transformation while vectors 5 and 6 for apple transformation. T-DNAs contained the CRISPR/Cas9 system driven by a constitutive (Arabidopsis thaliana Ubiquitin-10 Promoter, Ubq10At-P) or an inducible (Heat Shock-Promoter, HS-P) promoter. For grapevine, the editing targets were the powdery mildew susceptibility gene VvMLO7 and the L-idonate dehydrogenase gene VvIdnDH. For apple, the editing targets were the fire blight susceptibility genes MdDIPM1 and MdDIPM4. T-DNAs were specifically designed to be self-excisable by using two different excision systems: (i) the FLP (Flippase)/FRT (Flippase Recognition Target site) recombination system and (ii) the CTS (Cleavage Target Site) recognized by the CRISPR/Cas9 system. Left and Right Borders (LB and RB); Cauliflower Mosaic Virus 35S Promoter (35S-P); Neomycin phosphotransferase II (nptII); E9 Terminator (E9-T); NopalinE Synthase Terminator (NOS-T); Crispr associated protein 9 wild-type (Cas9 WT); Arabidopsis thaliana U6 Promoter (U6At-P); guide RNA for the CRISPR/Cas9 system (gRNA); short hairpin to detach RNA-polymerase from DNA strand (STOP).