Table 3.
Performance assessment of the NGS method for the detection of T-DNA insertion points (PoI) in the plant genome.
| Lines | Point of integration (PoI) | Total Reads | No. of clusters | Cluster size of true PoI | Cluster sequence length (bp) |
|---|---|---|---|---|---|
| (Chr. position) | |||||
| GT92.2 | Chr.14 pos 27434537 | 136,738 | 2 | 69 | 497 |
| GT103.1 | Chr. unknown pos 10269745 | 97,064 | 1 | 71 | 484 |
| GT109.3 | Chr.6 pos 14343411 | 87,945 | 1 | 124 | 502 |
| GT110.4 | Chr.4 pos 20705483 | 114,260 | 2 | 24 | 481 |
| GT110.5 | Not found | 57,334 | 1 | 53 | 487 |
| GT110.11 | Chr.18 pos 1299053 | 83,312 | 1 | 30 | 476 |
| GT110.15 | Chr.8 pos 17161782 | 116,919 | 2 | 23 | 450 |
| GT110.18 | Chr.19 pos 2014635 | 141,650 | 1 | 176 | 499 |
| GT110.20 | Chr.11 pos 6359640 | 134,784 | 1 | 17 | 403 |
| V1-4 | Chr.05 pos 47471717 | 201,962 | 2 | 30 | 497 |
| V1-10 | Chr.09 pos 36515162 | 180,599 | 1 | 133 | 487 |
| V1-14 | Chr.09 pos 10696063 | 62,637 | 1 | 76 | 502 |
| V2-3 | Chr.00 pos 17491423 | 91,574 | 3 | 758 | 502 |
| V4-5 | Chr.11 pos 5475365 | 136,756 | 1 | 13 | 486 |
| V4-27 | Chr.13 pos 3727444 | 174,867 | 3 | 464 | 501 |
| V4-34 | Chr.01 pos 28464453 | 123,321 | 2 | 25 | 490 |
| V6-2 | Chr.11 pos 9769022 | 88,950 | 3 | 10 | 475 |
| V6-10 | Chr. 5 pos 45547583 | 72,779 | 2 | 32 | 453 |
Chr., Chromosome; n., number.