Figure 4.

Application of the alternation reconstruction method on PC3 non-adherent cells at low density. Estimated number of cells $\approx 8450$, corresponding to a volumetric density of $\approx 14\times {10}^6$ cells/ml (measured in a $20\mathrm{\mu }\text{m}$ thick chamber) or a surface density of $\approx 290$ cells/${\text{mm}}^2$. (a) Full field of view, final reconstruction. (b) Reconstructions of 6 selected regions of interest from image (a) and comparisons with their fluorescence microscope acquisitions. (c–g) Reconstruction of the seventh region of interest, with corresponding raw acquisition (c), first (old) reconstruction result (d), CNN output result (e), final (new) reconstruction result (f) and the comparison with the fluorescence acquisition (g). (h) OPD profiles through one cell (red line 1 in (b)). The maximum OPD on the final reconstruction is about 1000 nm which corresponds to a phase shift of about 5$\pi$. (i) OPD profiles through two cells (red line 2 in (b)). (j) OPD profile through two cells (red line in (f)). The black arrows indicate CNN errors (orange curve in (j) and red arrows in (e)) that are corrected by the last reconstruction (green curve).