Figure 5.

Application of the alternation reconstruction method on PC3 non-adherent cells at high density. Estimated number of cells: $\approx 34,000$ corresponding to a volumetric density of $\approx 57\times {10}^6$ cells/ml (measured in a $20\mathrm{\mu }\text{m}$ thick chamber) or a surface density of $\approx 1140$ cells/${\text{mm}}^2$. (a) Full field of view final reconstruction. (b) Reconstructions of 6 selected regions of interest (a) and their comparisons with the fluorescence microscope acquisitions. (c–g) Reconstruction of the seventh region of interest, with corresponding raw acquisition (c), first (old) reconstruction result (d), CNN result (e), final (new) reconstruction result (f) and the comparison with the fluorescence acquisition (g). Red boxes in (e–g) highlight the discrepancies between the two modalities.