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. 2020 Nov 19;10:20207. doi: 10.1038/s41598-020-76411-9

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Results of the alternation reconstruction method applied to adherent PC12 cells treated with neuron growth factor. The first reconstruction results is shown in (a), CNN result in (b) and final reconstruction result in (c). (d–o) present detailed results corresponding to three regions of interest depicted by red boxes in (a). (g) OPD profile through two neuron cell bodies (red line in (d)). (k) OPD profile through two dendrites (red line in (h)). (o) OPD profile measured at the proximity of a cell, through two dendrites (red line in (l)).