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. 2020 Nov 19;10:20237. doi: 10.1038/s41598-020-77281-x

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Effect of 11β-HSD1 knockdown on TSLP production in NHEK cells. (a) We transfected NHEK with either 11β-HSD1 siRNA or negative siRNA then mRNA expression of 11β-HSD1 was quantified by qPCR after six and 24 h incubations with poly I:C and IL-4. (b) Cortisol in culture medium measured after six-hour incubation with poly I:C and IL-4. (c) Amount of TSLP in culture media measured after six and 24 h incubations with poly I:C and IL-4. (d) mRNA expression of TSLP quantified by qPCR after irradiation with 0, 20, 50, 100 mJ/cm² UVB. (e) Amount of TSLP measured after irradiation with 0, 20 and 50 mJ/cm² UVB (*p < 0.05, ** p < 0.005). Data are presented as means ± standard deviation. IL-4, interleukin-4; poly I:C, polyinosinic:polycytidylic acid; siRNA, small interfering RNA; UVB, ultraviolet B.