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. 2020 Nov 19;10:20225. doi: 10.1038/s41598-020-77287-5

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Silencing HNF1α gene expression inhibits proliferation and induces apoptosis in PDAC. (A) Expression levels of pAKT, AKT, PP13K, PI3K, pERK, ERK, pmTOR, mTOR, pP70S6K, and P70S6K were determined by western blot in silenced HNF1α AsPC-1 cells. (B) Immunofluorescence analysis showed cell proliferation markers for pAKT in siHNF1α AsPC-1 cells at × 100 magnification. (C,D) Apoptosis analysis of siHNF1α AsPC-1 cells measured by flow cytometry. (E) Western blot analysis of apoptotic markers in siHNF1α AsPC-1 cells. (F) Immunofluorescence analysis of BCl2 in siHNF1α AsPC-1 cells at × 100 magnification. Data shown as mean ± SEM. Experiments (n = 3) were repeated three times in triplicates. *p < 0.05.