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. 2020 Nov 19;10:20225. doi: 10.1038/s41598-020-77287-5

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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HNF1α regulates metastatic characteristics of PDAC. (A,B) Wound-healing assay was performed in siHNF1α AsPC-1 cells; migration was analyzed using Nikon Biostation CT at 2 h intervals for up to 96 h at × 4 magnification. (C,D) Invasiveness of siHNF1α AsPC-1 cells and overexpression HNF1α HPAC cells were observed using a Matrigel invasion assay, and captured using Nikon Eclipse TS 100 microscope at × 20 and × 100 magnification. (E,F) Colony formation assay was performed in siHNF1α AsPC-1 cells. (G) EMT markers analyzed by western blot in siHNF1α AsPC-1 cells. (H) Immunofluorescence analysis of Snail in siHNF1α AsPC-1 cells, captured using Nikon SMZ 1500 microscope at × 40 magnification. Data shown as mean ± SEM. Experiments (n = 3) were repeated three times in triplicates. *p < 0.05.