Figure 2.

Sex-dependent Cyp2a5 promoter methylation across three time points (E17.5, D3 mice and adult offspring). DNA was isolated from whole liver of foetuses (E17.5, a), neonatal offspring (D3, b) and adult offspring (c) in PSE (closed symbols) and control groups (open symbols). DNA was subjected to bisulphite sequencing-based methylation analysis of the Cyp2a5 promoter region and the percentage of DNA methylation was assessed. Data of the 6 targeted CpG-sites are presented per sex and exposure as individual values with median as a horizontal line. CpG-site annotations are relative to the ATG start codon. If not stated otherwise, the comparison of shown groups was not significant. *p ≤ 0.05, ** p ≤ 0.01, ***p ≤ 0.001 (Mann-Whitney U-test). Circle (○) symbol(s) = male, square (□) symbol(s) = female