Table 1.

Sequences of primers used in bisulphite-based methylation analysis

Gene	Targeted CpG-sites	Sequence 5ʹ-3’
Cyp2a5	CpG-614 to −542	For: TTTGTGTTTGTTTTGAGTGTTGGGATTA Rev: CCCCATCCACAACCATTCTT Seq: GTTGGGATTATAGGTTTATATTA Sequence to analyse: TTATATTYGATTTTTGGGAGTTTTTTAATGAAGAGGATTTTGAATTTAAGGATGYGAGAA GTGGAGATTT TAGGGTTATYGG
	CpG-74 to +45	For: AGTGGATAGTTTGGAGGTGAAAT Rev: ACAACTTTCCTAAAAACTTTCTCTACTTC Seq1: GGAGGTGAAATAGTTGTATAATTAA Seq2: TAGTTATTATTGTTTGTTTATTAT Sequence to analyse: S1: GATTAAAGTTYGTTTTTTTGTTTTTGGATGTATAAAAGTAAGTTAATT S2: TTATYGTTATTATGTTGATTTTAGGATTTTTTTTGGTGGTTGTAGTGGTTTTTTTTAGYGTTTTGGTTTT