Table 3.

Correlations between cotinine levels, Cyp2a5 mRNA expression and promoter methylation in foetal (E17.5), neonatal (D3) and adult offspring liver

			E17.5
correlation of / with	Cotinine formation (nmol/min/mg)		all	all male	all female	all control	all PSE	male control	male PSE	female control	female PSE
Cyp2a5 (2-dCT)		r	0.45	0.47	0.41	0.57	0.33	0.41	0.46	0.78	0.20
		p-value	0.00**	0.03*	ns	0.01**	ns	ns	ns	0.01**	ns
Cyp2a5 promoter methylation [%]	CpG-614	r	-0.15	-0,21	-0.14	0.30	-0.07	-0.41	0.19	0.44	-0.27
		p-value	ns	ns	ns	ns	ns	ns	ns	ns	ns
	CpG-589	r	-0.59	0.01	-0.10	0.30	-0.05	0.07	0.28	0.65	-0.35
		p-value	ns	ns	ns	ns	ns	ns	ns	ns	ns
	CpG-542	r	0.15	0.17	0.03	0.36	-0.09	0.55	-0.18	0.87	-0.17
		p-value	ns	ns	ns	ns	ns	ns	ns	0.00***	ns
	CpG-74	r	0.21	0.31	0.10	0.12	0.26	0.36	0.30	-0.03	0.06
		p-value	ns	ns	ns	ns	ns	ns	ns	ns	ns
	CpG-7	r	0.14	0.26	0.01	0.54	-0.19	0.42	0.08	0.75	-0.38
		p-value	ns	ns	ns	0.02*	ns	ns	ns	ns	ns
	CpG+45	r	0.31	0.34	0.28	0.4	0.37	0.19	0.57	0.57	0.21
		p-value	ns	ns	ns	ns	ns	ns	ns	ns	ns

			D3
correlation of / with	Cotinine formation (nmol/min/mg)		all	all male	all female	all control	all PSE	male control	male PSE	female control	female PSE
Cyp2a5 (2-dCT)		r	0.16	0.53	-0.12	0.01	0.21	0.21	0.81	0.14	-0,18
		p-value	ns	ns	ns	ns	ns	ns	0.01**	ns	ns
Cyp2a5 promoter methylation [%]	CpG-614	r	-0.10	0,46	-0.63	0.14	-0.30	0.59	0.21	-0.43	-0.79
		p-value	ns	ns	0.00***	ns	ns	ns	ns	ns	0.01**
	CpG-589	r	-0.14	0.48	-0.63	0.01	-0.28	0.69	0.20	-0.61	-0.59
		p-value	ns	ns	0.00***	ns	ns	ns	ns	ns	ns
	CpG-542	r	-0.26	-0.38	-0.42	-0.18	-0.26	0.20	-0.28	-0.54	-0.17
		p-value	ns	ns	ns	ns	ns	ns	ns	ns	ns
	CpG-74	r	-0.29	-0.01	-0.29	-0.57	-0.12	-0.03	-0.28	-0.36	0.18
		p-value	ns	ns	ns	ns	ns	ns	ns	ns	ns
	CpG-7	r	0,47	0,70	0.30	0.38	0.29	0.46	0.83	0.32	0.18
		p-value	ns	0.00***	ns	ns	ns	ns	0.00***	ns	ns
	CpG+45	r	0.19	0,77	-0.52	0.12	0.21	0.80	0.86	-0.64	-0.44
		p-value	ns	0.00***	ns	ns	ns	ns	0.00***	ns	ns

			Adult
correlation of / with	Cotinine formation (nmol/min/mg)		all	all male	all female	all control	all PSE	male control	male PSE	female control	female PSE
Cyp2a5 (2-dCT)		r	0.23	0.26	0.21	0.67	-0.67	0.21	-0.37	0.47	-0.41
		p-value	ns	ns	ns	0.00**	ns	ns	ns	ns	ns
Cyp2a5 promoter methylation [%]	CpG-614	r	-0.24	-0.42	-0.12	0.02	-0.75	-0.50	0.30	0.42	-0.89
		p-value	ns	ns	ns	ns	0.01**	ns	ns	ns	ns
	CpG-589	r	0.04	0.05	-0.09	0.24	-0.35	-0.15	-0.78	0.07	-0.53
		p-value	ns	ns	ns	ns	ns	ns	ns	ns	ns
	CpG-542	r	-0.37	-0.37	-0.21	-0.33	-0.51	-0.16	0.00	0.28	-0.90
		p-value	ns	ns	ns	ns	ns	ns	ns	ns	ns
	CpG-74	r	-0.19	-0.18	0.64	-0.28	0.73	-0.17	-0.52	0.48	0.90
		p-value	ns	ns	0.01**	ns	0.01**	ns	ns	ns	ns
	CpG-7	r	0.017	0.21	0.01	-0.17	0.55	0.19	-0.41	-0.22	0.89
		p-value	ns	ns	ns	ns	ns	ns	ns	ns	ns
	CpG+45	r	-0.03	0.23	0.21	-0.15	0.31	0.65	-0.20	0.03	0.80
		p-value	ns	ns	ns	ns	ns	ns	ns	ns	ns

*p ≤ 0.05, ** p ≤ 0.01, ***p ≤ 0.001. P-values were adjusted by the Bonferroni method.