Figure 3.

PIWIL1 is the target of miR-2116-3p

A. The expression of potential target genes was assessed by qRT-PCR in LoVo and HCT8 cells after overexpressing miR-2116-3p. B. The expression level of PIWIL1 in CRC cell lines and normal NCM460 controls was determined by qRT-PCR. C. Putative binding sites between miR-2116-3p and PIWIL1 predicted by TargetScan. D. Dual luciferase reporter assays were performed in LoVo and HCT8 cells to determine the physical interaction between miR-2116-3p and PIWIL1. E. RIP assays revealed the significant enrichment of FALEC, miR-2116-3p and PIWIL1 by anti-Ago2. F. qRT-PCR was conducted to study the expression of PIWIL1 in CRC cells with FALEC silence or together with miR-2116-3p inhibition. **P < .01.