Figure 2.

Effects of interaction of the 3´UTR of SIRT6 mRNA with miR-186-5p. (a) Co-transfection of the pmirGLO_SIRT6 vector containing the full-length 3´UTR of SIRT6 mRNA and the firefly luciferase reporter gene with pre-miR-186-5p results in a significant decrease in luminescence as compared to co-transfection with the pre-neg-miR negative control (neg1). Results are presented as mean with standard deviation. Statistical analysis was performed with a two-tailed Student t-test. (b) Transfection of HEK293 cells with pre-miR-186-5p results in a decrease in the expression of endogenous SIRT6 protein. Hsp90 – heat shock protein 90, control protein expression of which is not affected by miR-186-5p. The Western blot analysis was performed once, and the results of densitometric scanning are provided in Supplementary Table S2