Figure 3.

Gli2 induces IGF-II expression to promote Huh-7 cell migration and invasion

(A-C) Huh-7 cells were transfected with sh-Gli2, and the levels of Gli2 and IGF-II mRNA were measured by qPCR (A-B); the levels of Gli2 and IGF-II protein were measured by western blotting (C). (D-E) The migration and invasion abilities of Huh-7 cells were measured by Transwell assay. (F-G) The migration of Huh-7 cells was confirmed by scratch assay. (H) The protein levels of E-cadherin, vimentin and MMP-9 were detected by western blotting. *** p < .001, ** p < .01, * p < .05.