Figure 2.

Knock-down of FGF13 inhibits A549 cells proliferation.A549 cells were transfected with siRNA-FGF13 or N.C siRNA, (a) the interference efficiency and relative mRNA expression levels of FGF13 were measured by real-time qPCR. (b) The protein expression level of FGF13 with the interference efficiency was detected by Western blotting. (c) Cell proliferation of A549 was measured by CCK8 assay at 0, 24, 48, 72 h post-transfection, respectively. (d) Cell colony formation was performed. (e) The number of A549 cells proliferation was checked by Ki67. (f) FGF13-silenced cells were harvested and samples were analyzed on the flow cytometer 72 h post-transfection. The cell numbers of G1 phase and S phase were counted in (f). Results are presented as mean± S. E. M. n = 3. *P < .05, ** P < .01