Figure 3.

FGF13 accelerates cell cycle progression and promotes proliferation in A549 cells. A549 cells were transfected with pcDNA3.1-FGF13 (or Empty vector), (a) the over-expression efficiency and relative mRNA expression levels of FGF13 were measured by real-time qPCR. (b) the FGF13 relative protein levels of over-expression efficiency were detected by western blot after 48 h transfection. (c) Cell proliferation of A549 was measured by CCK8 assay at 0, 24, 48, 72 h post-transfection, respectively. (d) Cell colony formation was performed, (e) cell cycle progression was analyzed by the flow cytometer. The cell numbers of G1phase and S phase were counted in (e). Results were presented as mean± S. E. M. n = 3. *P < .05, ** P < .01