Figure 5.

Interaction between FGF13 and SHCBP1 affects cell proliferation. FGF13 affects AKT signaling pathway, p21 and p27 expression levels were detected in A549 cells which overexpressed or interfered. (a) The mRNA expression levels of p21, p27 and CDK2 were analyzed on A549 cells with FGF13 silenced, the protein expression of p21, p27, cyclinE1 and FGF13 were detected with FGF13 silenced, respectively. (b) The mRNA expression levels of p21, p27 and CDK2 were evaluated in A549 cells with FGF13 overexpressed, the protein expression of p21, p27, cyclinE1 and FGF13 were detected with FGF13 overexpressed. (c) After FGF13 and SHCBP1 were silenced, the western blotting was employed to assess the expression of p21, p27 and cyclinE1, β-actin was used as a loading control. (d) FGF13 was overexpressed while SHCBP1 was interfered in A549 cells, the protein expression levels of p21, p27 and cyclinE1 were analyzed. The results of western blotting were assessed using ImageJ (mean ± SD of 3 independent experiments.*P < .05, ** P < .01)