Figure 4.

LRRC75A-AS1/miR-199b-5p/PDCD4 axis functioned in MM. (a) The inference efficiency of shPDCD4 in U266 cells for rescue assays was analyzed through both qRT-PCR and western blot. (b-c) MM cells were under transfection of pcDNA3.1, pc-LRRC75A-AS1, pc-LRRC75A-AS1 + miR-199b-5p mimics, or pc-LRRC75A-AS1 + shPDCD4. The proliferation of U266 cells in four groups was dissected via colony formation and EdU assays. (d-f) The apoptosis of U266 cells under four treatments was estimated via TUNEL, flow cytometry and western blot. **P < .01 vs control group