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. 2020 Nov 20;15(11):e0241306. doi: 10.1371/journal.pone.0241306

Fig 1. Bioinformatic workflow.

Fig 1

From the 1,577 B. pseudomallei genomes found on NCBI, 511 were unique and associated with disease and these were used for further analysis. The pangenome of these 511 genomes comprised 19,991 unique genes. 4,496 of these were classified as core genes. Predicted translation of these genes gave 726 predicted extra-cytoplasmic proteins. Of these extra-cytoplasmic proteins, 263 were predicted to contain an even number of cysteines. We predict that these 263 proteins are substrates of B. pseudomallei DsbA.