Fig 2. Phylogenetic tree of KV channels in fungi, and representative animal and plant species.
The Shaker-like K+ channel tree of publicly available sequenced fungi from the Basidiomycota phylum and from basal fungi (Cryptomycota, Chytridiomycota, Blastocladiomycota, Zoopagomycota and Mucoromycota) was calculated by the maximum likelihood method (1000 bootstraps). Shaker-like proteins were found only in one putative Ascomycota species (Saitoella complicata) and not in the Pucciniomycotina and Ustilaginomycotina subphyla of Basidiomycota. Ectomycorrhizal (ECM), arbuscular mycorrhizal (AM), and orchid mycorrhizal (OM) fungi are indicated by yellow, green, and blue dots, respectively. KV channels from representative land plant and animal species were included in the tree. All species displayed in this tree are listed in the S2 Table. Shaker-like proteins from H. cylindrosporum (HcSKC) and L. bicolor (LbSKC) used in this study are indicated by black arrows.