HU does not change mRNA and protein expression of glutamatergic receptors. (A) mRNA expression of NMDAR subunits (GluN1, GluN2A, GluN2B, GluN2C, GluN2D, GluN3A, GluN3B) relative to B2m. Values are normalized to control rats (“1”, represented by dashed line). (B) Representative immunoblot for NR1, GluA1 and tubulin of two control and two HU individual nTS samples. (C, D) Quantification of NR1 and GluA1 protein expression, relative to tubulin, in the nTS of control and HU rat. Values are normalized to control rats (“1”). Data are shown as mean±SD.