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. Author manuscript; available in PMC: 2021 Nov 21.
Published in final edited form as: Neuroscience. 2020 Oct 9;449:214–227. doi: 10.1016/j.neuroscience.2020.09.043

Fig. 8.

Fig. 8.

Hyperpolarization of HU membrane potential is mediated by inhibitory synaptic inputs on second-order nTS neurons. (A) Mean data showing the effects of progressive neurotransmission blockade (AP5 10 μM; NBQX 10 μM; GBZ 25 mM) on RMP of control (black) and HU (red) group. Synaptic blockade produced no changes in RMP of control cells. By contrast, whereas RMP of HU neurons was not affected by glutamatergic receptors blockade, antagonism of GABAergic neurotransmission (GBZ) significantly depolarized these cells. (B) Average data illustrating the number of action potentials evoked by step-depolarization after complete synaptic blockade. Note that under this condition, the firing rate is comparable between control and HU. #p<0.05 Gabazine vs ACSF, one-way RM-ANOVA test.