Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference |
Identifiers | Additional information |
|---|---|---|---|---|
| Genetic reagent (Mus musculus) | C57BL/6J | Charles River | C57BL/6NCrl | |
| Genetic reagent (Mus musculus) | C57BL/6J.LRRC8AEm1Uman
(Lrrc8afl/fl) |
This paper | Line maintained by David Brough lab, University of Manchester | |
| Genetic reagent (Mus musculus) | B6J.B6N(Cg)-Cx3cr1tm1.1(cre)Jung/J | Jackson lab | Stock No: 025524 RRID:IMSR_JAX:025524 |
Obtained from breeding colony managed by John Grainger lab (University of Manchester) |
| Cell line (Mus musculus) | Bone-marrow-derived macrophages (BMDMs) | In house | Generated from bone marrow from above mouse lines | |
| Cell line (Mus musculus) | Peritoneal macrophages | In house | Generated by peritoneal lavage from above mouse lines | |
| Cell line (Homo Sapien) |
HeLa | ATCC | HeLa (ATCC CCL-2) RRID:CVCL_0030 | |
| Antibody | Anti-mouse IL-1β (goat polyclonal) | R and D Systems | AF-401-NA RRID:AB_416684 |
(1:500) |
| Antibody | Anti-Caspase1 + p10 + p12 (rabbit monoclonal) | Abcam | ab179515 | (1:1000) |
| Antibody | Anti- mouse GSDMD (rabbit monoclonal) | Abcam | ab209845 RRID:AB_2783550 |
(1:1000) |
| Antibody | Anti-mouse ASC/TMS1 (D2W8U) (rabbit monoclonal) | Cell Signaling Technology | 67824 RRID:AB_2799736 |
(1:1000) |
| Antibody | LRRC8A (8H9) (mouse monoclonal) | Santa Cruz | sc-517113 | (1:200) |
| Antibody | Anti-β-Actin−Peroxidase (mouse monoclonal) | Sigma | A3854 RRID:AB_262011 |
(1:20000) |
| Antibody | Anti-Rabbit Immunoglobulins HRP (goat polyclonal) | Agilent | P044801-2 RRID:AB_2617138 |
(1:1000) |
| Antibody | Anti-Mouse Immunoglobulins HRP (rabbit polyclonal) | Agilent | P026002-2 RRID:AB_2636929 |
(1:1000) |
| Antibody | Anti-Goat Immunoglobulins HRP (rabbit polyclonal) | Agilent | P044901-2 | (1:1000) |
| Recombinant DNA reagent | pcDNA3.1 Hygro EYFP H148Q/I152L | Addgene | 25874 RRID:Addgene_25874 |
A gift from Peter Haggie |
| Commercial assay or kit | CytoTox 96 Non-Radioactive Cytotoxicity (LDH) Assay | Promega | G1780 | |
| Commercial assay or kit | Mouse IL-1β/IL-1F2 DuoSet ELISA | R and D systems | DY401 | |
| Chemical compound, drug | Lipopolysaccharides from Escherichia coli O26:B6 | Sigma | L2654 | For in vitro experiments |
| Chemical compound, drug | Lipopolysaccharides from Escherichia coli O127:B8 (in vivo) | Sigma | L3880 | For in vivo experiments |
| Chemical compound, drug | Adenosine Triphosphate (ATP) | Sigma | A2383 | |
| Chemical compound, drug | Nigericin sodium salt | Sigma | N7143 | |
| Chemical compound, drug | Silica | U.S. Silica | MIN-U-SIL 15 | |
| Chemical compound, drug | Imiquimod | InvivoGen | R837 | |
| Chemical compound, drug | Tamoxifen | Sigma | T5648 | |
| Chemical compound, drug | 4-Sulfonic calix[6]arene Hydrate | Thermo Fisher | 10494735 | |
| Chemical compound, drug | 4-[(2-Butyl-6,7-dichloro-2-cyclopentyl-2,3-dihydro-1-oxo-1H-inden-5-yl)oxy]butanoic acid (DCPIB) | Tocris | 1540 | |
| Chemical compound, drug | Flufenamic acid (FFA) | Sigma | F9005 | |
| Chemical compound, drug | NS3728 | David Brough Lab, University of Manchester | ||
| Chemical compound, drug | CP-456773 sodium salt (MCC950) | Sigma | PZ0280 | |
| Chemical compound, drug | NBC19 | David Brough Lab, University of Manchester | PMID:28943355 | |
| Chemical compound, drug | Calcein, AM, cell-permeant dye | Thermo Fisher | C1430 | |
| Chemical compound, drug | Disuccinimidyl suberate (DSS) | Thermo Fisher | 21555 |