Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Gene (Drosophila melanogaster) | Ci | Flybase ID: FBgn0004859 |
CG2125 | |
| Gene (Drosophila melanogaster) | Cos2 | Flybase ID: FBgn0000352 |
CG1708 | |
| Gene (Drosophila melanogaster) | PKA | Flybase ID: FBgn0000273 |
CG4379 | |
| Gene (Drosophila melanogaster) | Fused | Flybase ID: FBgn0001079 |
CG6551 | |
| Gene (Drosophila melanogaster) | Suppressor of Fused | Flybase ID: FBgn0005355 |
CG6054 | |
| Genetic reagent (Drosophila melanogaster) | hs-flp | PMID:7867064 | FBti0002738 | hsp70-driven Flp recombinase on X |
| Genetic reagent (Drosophila melanogaster) | ci94 | PMID:7705626
PMID:10102270 |
FBal0045443 | 5 kb deletion removing promoter and first exon |
| Genetic reagent (Drosophila melanogaster) | ciCe | PMID:10102270 | ciCe2 FBal0001657 | 8 bp deletion that is expected to result in a truncation of the protein at amino acid residue 975 |
| Genetic reagent (Drosophila melanogaster) | Dp[y+] | PMID:10102270 | Dp(1;4)1021[y+] svspa-pol FBab0003151 | |
| Genetic reagent (Drosophila melanogaster) | Su(fu)LP | PMID:1468628 | FBal0016296 | Amorphic 1.5 kb deletion extending into neighboring kar gene |
| Genetic reagent (Drosophila melanogaster) | pka-C1H2 | PMID:8391504 | FBal0033960 | G203D alteration to key kinase domain residue |
| Genetic reagent (Drosophila melanogaster) | smo2 | PMID:15592457 | FBal0015765 | Behaves as a null |
| Genetic reagent (Drosophila melanogaster) | FRT 42D P[Smo+] | PMID:10102270 | P[Smo+, hsp70-GFP] FBtp0012072 | Fully rescues loss of smo function |
| Genetic reagent (Drosophila melanogaster) | FRT 42D cos22 | PMID:11090136 | FBal0001772 | To generate loss-of-function cos2 clones |
| Genetic reagent (Drosophila melanogaster) | fumH63 | PMID:8846897 | FBal0120493 | G203D loss of kinase activity |
| Genetic reagent (Drosophila melanogaster) | tub-GAL80 FRT 40A | BDSC BL-5192 | For MARCM clones on 2L | |
| Genetic reagent (Drosophila melanogaster) | FRT 42D P[Ci+] tub-GAL80 | PMID:10102270 | P[Ci+] 16 kb segment rescues ci null in stock for 2R MARCM clones | |
| Genetic reagent (Drosophila melanogaster) | C765 > Gal4 | Flybase ID: FBti0002765 |
Spatially uniform wing disc GAL4 driver | |
| Genetic reagent (Drosophila melanogaster) | UAS-GAP-Fu | PMID:17658259 | FBal0284373 | Fu coding sequence with Myristoylation sequence from hGAP43 at N-terminus and CFP at C-terminus |
| Genetic reagent (Drosophila melanogaster) | ptc-lacZ | PMID:8898207 | P[ptcA-lacZ] FBal0047864 | 10.8 kb ptc promoter driving lacZ |
| Antibody | Anti-Ci-155 (rat monoclonal) | DSHB | AB_2109711 | (1:3) |
| Antibody | Anti-beta-galactosidase (rabbit polyclonal) | MP Biomedicals | AB_2334934 | (1:10,000) |
| Antibody | Anti-Engrailed (mouse monoclonal) | DSHB | AB_528224 | (1:5) |
| Antibody | AlexaFluor 488, 546, 594, 647 |
Thermofisher Scientific | Anti-rabbit, Anti-mouse Anti-Rat |
(1:1000) |
| Recombinant DNA reagent | pCFD4 | Mann Lab | Addgene: 83954 | Gibson cloning of gRNA |
| Recombinant DNA reagent | Bluescript genomic Cubitus interruptus | Basler Lab | ||
| Recombinant DNA reagent | att-Pacman Expression Vector | DGRC | ||
| Chemical compound, drug | Normal Goat Serum | Jackson Immunoresearch laboratories | RRID:AB_2336990 | |
| Chemical compound, drug | Aqua Polymount | PolySciences | CN: 18606–20 | |
| Commercial assay or kit | Gibson Assembly | New England Biolabs | CN: E5510S | |
| Commercial assay or kit | PfuUltraII Fusion HS DNA polymerase | Agilent Technologies | CN: 600670 | |
| Commercial assay or kit | Zero Blunt Topo cloning vector | Invitrogen | CN: K270020 | |
| Strain, strain background (Escherichia coli) | Transformax EPI 300 Electrocompetent E. coli | Epicentre Now lucigen |
CN: EC300110 | Electro-competent cells |
| Strain, strain background (Escherichia coli) | One Shot TOP10 Chemically Competent E. coli | Thermofisher Scientific | CN: C4040-10 | Chemically Competent cells |
| Software, algorithm | Image J | NIH Bethesda Maryland | ||
| Software, algorithm | A Plasmid Editor (APE) |