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. 2020 Nov 20;6(47):eabd0276. doi: 10.1126/sciadv.abd0276

Fig. 4. KSHV ORF33 recruits PPM1G to dephosphorylate STING and MAVS.

Fig. 4

(A) Coomassie Blue–stained gel of purified His-PPM1G and His-PPM1G D496A from bacteria. (B and C) The effect of His-PPM1G and His-PPM1G D496A on the phosphorylation levels of STING and MAVS was examined by in vitro phosphatase assay. (D and E) Interaction between endogenous PPM1G and exogenously expressed ORF33 (D) or ORF33 expressed from viral genome (E) was examined by coimmunoprecipitation (co-IP). (F to I) Enhancement of interaction between PPM1G and STING (F and G) or MAVS (H and I) by ORF33 was examined by co-IP. See also fig. S6.