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. 2020 Nov 20;6(47):eabc1204. doi: 10.1126/sciadv.abc1204

Fig. 6. Simultaneous detection of EV CD63 and PD-L1 membrane protein by FCM and detection of EV PD-L1 mRNA by RT-qPCR.

Fig. 6

(A) Double staining with CD63-AF488 and PD-L1–AF647 fluorescent antibodies of EVs in plasma from healthy donors and patients with LUAD. (B) EVs in plasma samples were captured with CD63 exosome capture beads and incubated with CD63-AF488 antibody, and FCM was used to detect the expression of EV membrane protein CD63. (C) EVs in plasma samples were captured with CD63 exosome capture beads and incubated with PD-L1–AF647 antibody, and FCM was used to detect EV membrane protein PD-L1 expression. (D) Statistic analysis of the differential membrane protein expression of PD-L1+ EVs in plasma from three healthy donors and six patients with LUAD. (E) Statistic analysis of the differential mRNA expression of PD-L1 in EVs from plasma of six healthy donors and six patients with LUAD.