. Author manuscript; available in PMC: 2020 Nov 20.

Published in final edited form as: Recent Results Cancer Res. 2020;215:89–104. doi: 10.1007/978-3-030-26439-0_5

Table 1.

CTC measurements automatically generated with the HD-SCA workflow

Feature	Description	Units
Physical characteristics	Physical measurements generated for each nuclei
x	Center of mass x (x-axis)	16-bit pixel value
y	Center of mass y (y-axis)	16-bit pixel value
Roundness	How closely the shape of an object approaches that of a circle
Circularity	Inverse of roundness
Area	Area size	16-bit pixel value²
Area (local ratio)	Ratio of the area of nuclei of interest to the average area of the surrounding 50 nuclei
Aspect ratio	Relationship between width and height
Solidity	Texture
Cluster count	Individual cell nuclei within a CTC aggregate	Cell nuclei
Expression-level measurements	Signal intensities generated for each cell, in each fluorescent channel (DAPI, TRITC, FITC, Cy5)
Mean	Mean pixel intensity of the cell of interest	16-bit pixel value
Standard deviation	Standard deviation of the mean pixel intensities of the cell of interest and the surrounding cells	16-bit pixel value
Standard deviation over the mean	The number of standard deviations over the mean pixel intensity for the cell of interest to the mean pixel intensity of the surrounding 50 cells	16-bit pixel value