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(a) System design for PRF based two-photon laser scanning fluorescence microscope. Galvo1, single axis galvo scanner (8315k Cambridge Technology); L1-6, achromatic telecentric relay lenses with focal length 125, 300, 300, 187.5, 125, and 500 mm; Galvo2, two-axis galvo scanner (8315k Cambridge Technology); BP, bandpass filter (535/150 Semrock). (b) Defocus distance’s dependence on galvo 1 voltage.
