Figure 1.

Microfluidic device design and overview. (a) Proposed application and device functionality. Micrographs of a mouse glioma tumor slice exposed to two different cell nuclear binding agents (Hoechst, blue, and Sytox Green, green) through alternating streams. (b) Cross-sectional schematic of the device. The device is operated by gravity flow and the total flow rate is driven by a syringe pump through a common outlet: one syringe pump controls flow across all 40 fluidic streams. Tissue slices are cultured on a PTFE porous membrane. The wet membrane seals the roofless microchannels by capillarity, which allows for fluidic stream transport of culture medium to tissue. (c) Exploded schematic of the PMMA platform showing from top to bottom: 1) bottomless plate with 40 inlet wells, 2) 300μm-thick channel network layer, and 3) 125 μm-thick sealing layer.