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Microfluidic device with Transwell insert. (a) Photograph of standard six-well plate with Transwell insert containing U87 GBM flank xenograft slices. (b) Live imaging with the platform with incorporated Transwell insert. (c) Fluorescent image showing microfluidic delivery of Hoechst (blue), Cell Tracker Green (green), and Cell Tracker Orange (red) to live GBM slices. (d) Fluorescent image showing fixed tissue 48-hours after delivery.
