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General overview of device fabrication protocol. The overall fabrication of the device consists of (a) laser cutting/engraving and cleaning each of the laminates that compose the microfluidic platform (30 min); (b) chloroform vapor exposure (6 min); (c) thermal fusion bonding (5 min); (d) solvation of the under surface of the 40-well plate with methylene chloride (1 min); (e) alignment and assembly by pressing (5 min); and outlet/base installation (10 min, step not shown).
