Fig. 2.

(a) Schematic illustration for the working principle of All-In-One Dual CRISPR-Cas12a (AIOD-CRISPR) assay: demonstrating the stimulation of RPA amplification to reveal the binding sites of Cas 12a-crRNA which turns on the fluorescence on activation of the endonuclease enzyme. (b) Designing and analysing of AIOD-CRISPR assay: The ssDNA-FQ reporter molecule was first marked with the fluorophore-5′6-FAM and a quencher, which was subsequently put through RPA reactive treatments: (i) the reaction system showing direct visualisation of bright fluorescence under LED, blue light, and UV light; (ii) the reactive system number 5 produces highest bright signal due to the presence of smaller DNA sizes cleaved from the reporter molecule; (iii) graph indicating the saturation of highest produced fluorescent signal in 13 min [94]. Reproduced with permission